Metagenomics sequencing

Service Introduction

Metagenomics sequencing is the high-throughput sequencing of the genomes of microbial communities in a specific environmental sample. It is used to analyze the composition and function of microbial communities, to understand the diversity and abundance of microbial communities, to explore the relationship between microbes and the environment, and to discover and study new genes with specific functions.

Based on high-throughput sequencing, metagenomics research has the following advantages:

Many microorganisms in the natural world cannot be cultured in the laboratory, thus expanding the scope of microbial research without the need for isolation and cultivation.

The method studies the macroscopic features of microbial community diversity, functional genes, etc. in the environment, which more quickly, efficiently, and accurately reflects the true state of microorganisms.

Without the need for enrichment of genomic fragments in host bacteria, high-throughput sequencing can analyze and explore the species diversity and functional genes in the total microbial community, promoting the wide application of metagenomics in a variety of research fields.

Platform advantages

Short cycle: After receiving customer samples, the cycle from quality inspection to the end of bioinformatics analysis is generally controlled within 30-45 working days, and the completion cycle will be adjusted according to the number of samples in the project.

Experienced technical staff: Can provide metagenomics and multi-omics experimental plans according to the requirements of partners, and solve experimental problems.

Technical Procedures

Bioinformatics Analysis

生物信息學分析
宏基因組細菌多樣性測序分析內容	宏基因組學分析內容
總體數據統計分析	將 reads 與已測序微生物基因組（或參考序列）進行比對，得到物種分類信息以及每個物種上的 reads 個數統計
單個樣品中微生物種類及豐度分析，序列聚類 OTU，群落結構分析	對 reads 組裝，得到 contigs 序列，根據 contig 的拼接結果，進行基因預測、基因功能注釋、COG 分類、GO 分類、KEGG-KO 分類及KEGG-map 代謝圖
多個樣品間比較分析，包括相似度比對，OTU 比較、多樣品群落結構分析，PCA（主成分）分析

Sample Requirements

實驗類型	樣品總量	樣品濃度	樣品純度OD260/280	DNA質量	樣品保存	樣品運輸
環境細菌多樣性	1ug DNA	>50 ng/μl	1.8-2.0,無蛋白質、RNA 或肉眼可見雜質污染	基因組完整、無降解，電泳結果基因組 DNA 主帶在預期位置，清晰，無彌散，cDNA 片段化樣品，片段大小符合預期位置	請選擇干粉、酒精、TE buffer 或超純水一種，并在樣品信息單中注明	請將樣品置于 1.5 ml 管中，做好標記，并用封口膜好，并同《測序樣品訂單》一起放在塑料樣品袋封口，基因組 DNA 如果用乙醇沉淀，可以常溫運輸。
宏基因組	3ug DNA	>30 ng/μl	1.8-2.0,無蛋白質、RNA 或肉眼可見雜質污染	基因組完整、無降解，電泳結果基因組 DNA 主帶在預期位置，清晰，無彌散，cDNA 片段化樣品，片段大小符合預期位置	請選擇干粉、酒精、TE buffer 或超純水一種，并在樣品信息單中注明