Small RNA sequencing
Service introduction
Small RNAs (sRNA) are important regulators of life activities, playing important roles in gene expression regulation, individual development, metabolism, and the occurrence of diseases. The Illumina sequencing platform can perform deep sequencing of all sRNA in the sample to achieve qualitative and quantitative research purposes. Each sample can obtain more than 10 million sRNA sequencing sequences. Through massive parallel sequencing, it can discover, identify, and quantify the small RNA map at the whole genome level of any species, the excavation of new mRNA molecules, the prediction of target genes, identification, and differential expression analysis between samples.
Technical procedures

Sample requirements
實驗類型 | RNA樣品總量 | 樣品濃度和純度 | 樣品質量 | 樣品保存 | 樣品運輸 |
Small RNA測序 | 一次性提供至少3 μg高質量的總 RNA | 樣品濃度 >100 ng/μl,OD260/280 介于 1.8-2.0 之間,無肉眼可見污染 | 使用 Agilent Bioanalyzer 儀器檢測,則獲得的總的 RNA 28S:18S >1,RIN>7.5 | 請選擇乙醇、DEPC 水保存樣品,并在樣品信息單中注明 | 樣品請置于 1.5 ml 管中,并使用封口膜封好,放置足夠的干冰運輸 |
Experiment cycle and sequencing strategy
實驗類型 | 測序策略 | 數據量 | 實驗周期 |
Small RNA測序 | HiseqSE50 | 10M | 樣品質檢合格后,30-50 個工作日 |

